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Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00652c
The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sF...
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creator | Safavi, A Emamzadeh, R Nazari, M Ehsani, M Zarkesh-Esfahani, S. H Rahgozar, S |
description | The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sFv to the C-terminus of super RLuc8. The results indicate that the probe can bind to CD4+ cells
via
its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.
A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super
Renilla
luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer. |
doi_str_mv | 10.1039/c6mb00652c |
format | article |
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via
its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.
A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super
Renilla
luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer.</description><identifier>ISSN: 1742-206X</identifier><identifier>EISSN: 1742-2051</identifier><identifier>DOI: 10.1039/c6mb00652c</identifier><language>eng</language><creationdate>2017-02</creationdate><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Safavi, A</creatorcontrib><creatorcontrib>Emamzadeh, R</creatorcontrib><creatorcontrib>Nazari, M</creatorcontrib><creatorcontrib>Ehsani, M</creatorcontrib><creatorcontrib>Zarkesh-Esfahani, S. H</creatorcontrib><creatorcontrib>Rahgozar, S</creatorcontrib><title>Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00652c</title><description>The human immunodeficiency virus (HIV) destroys CD4+ lymphocytes and monitoring these cells is one of the best techniques for studying HIV infection. In the present study a novel bioluminescent probe, super RLuc8-sFv, is developed in order to detect human CD4+ cells by fusion of an anti-human CD4 sFv to the C-terminus of super RLuc8. The results indicate that the probe can bind to CD4+ cells
via
its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.
A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super
Renilla
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via
its sFv domain; also it emits visible light through its signalling domain. Super RLuc8-sFv provides a new gateway for detection of human CD4+ cells using luminometric-based assays and may reduce the difficulties involved in, and the cost of, HIV-related diagnostic tests.
A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super
Renilla
luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer.</abstract><doi>10.1039/c6mb00652c</doi><tpages>6</tpages></addata></record> |
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title | Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cellsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6mb00652c |
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