Structure determination of microbial metabolites by the crystalline sponge method

The structures of metabolites produced in microgram quantities by enzymatic reductions with baker's yeast were analyzed using the crystalline sponge method. The X-ray data provided reliable structures for trace metabolites including their relative and absolute stereochemistries that are not ful...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2016-01, Vol.7 (6), p.391-3913
Main Authors: Inokuma, Yasuhide, Ukegawa, Tomoya, Hoshino, Manabu, Fujita, Makoto
Format: Article
Language:English
Subjects:
Chemistry
Chromatography
Crystal structure
Low level
Metabolites
Microorganisms
Purification
Saccharomyces cerevisiae
Sponges
X-rays
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Summary:The structures of metabolites produced in microgram quantities by enzymatic reductions with baker's yeast were analyzed using the crystalline sponge method. The X-ray data provided reliable structures for trace metabolites including their relative and absolute stereochemistries that are not fully addressed by conventional NMR and LC-MS analyses. Technically, combining two or more chromatographic purification techniques is essential because, unlike abundant synthetic compounds, extracted metabolites contain many low level UV-silent impurities. The crystalline sponge method coupled with HPLC purification (LC-SCD) would thus be a useful method for metabolic analysis and drug discovery. The structures of metabolites produced in microgram quantities by enzymatic reductions with baker's yeast were analyzed using the crystalline sponge method. The crystalline sponge method coupled with HPLC purification would be a useful method for metabolic analysis and drug discovery.
ISSN:2041-6520
2041-6539
DOI:10.1039/c6sc00594b