A fluorescent probe for bisulfite ions: its application to two-photon tissue imagingElectronic supplementary information (ESI) available. See DOI: 10.1039/c6tb02637k

A benzoxazinone based fluorescent probe for the specific and efficient detection of bisulfite ions in aqueous medium is described. The probe formed a bisulfite/sulphite adduct with an associated turn-on fluorescence response in the red wavelength region. No interference was observed in the detection...

Full description

Saved in:
Bibliographic Details
Main Authors: Agarwalla, Hridesh, Pal, Suman, Paul, Anirban, Jun, Yong Woong, Bae, Juryang, Ahn, Kyo Han, Srivastava, Divesh N, Das, Amitava
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A benzoxazinone based fluorescent probe for the specific and efficient detection of bisulfite ions in aqueous medium is described. The probe formed a bisulfite/sulphite adduct with an associated turn-on fluorescence response in the red wavelength region. No interference was observed in the detection process from all possible competing anions and molecules, including cyanide ion, cysteine, homocysteine and glutathione. In addition, the probe showed a fast response time, low detection limit, and cell membrane permeability. Furthermore, the probe was two-photon excitable, enabling imaging of endogenous bisulfite ions in HeLa cells as well as in deep tissues from different organs of mouse. Two-photon imaging of endogenous bisulphite ions of the hippocampus region of mouse brain.
ISSN:2050-750X
2050-7518
DOI:10.1039/c6tb02637k