A narrow open tubular column for high efficiency liquid chromatographic separation

We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow ( e.g. , 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2018-04, Vol.143 (9), p.28-211
Main Authors: Chen, Huang, Yang, Yu, Qiao, Zhenzhen, Xiang, Piliang, Ren, Jiangtao, Meng, Yunzhu, Zhang, Kaiqi, Juan Lu, Joann, Liu, Shaorong
Format: Article
Language:English
Subjects:
Elution
Isotopes
Liquid chromatography
Separation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow ( e.g. , 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with octadecylsilane and both isocratic and gradient separations are performed. We reveal a focusing effect that may be used to interpret the efficiency enhancement. We also demonstrate the feasibility of using this technique for separating complex peptide samples. This high-resolution and fast separation technique is promising and can lead to a powerful tool for trace sample analysis. We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow ( e.g. , 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar.
ISSN:0003-2654
1364-5528
DOI:10.1039/c7an02065a