Identification of actin as a direct proteomic target of berberine using an affinity-based chemical probe and elucidation of its modulatory role in actin assemblyElectronic supplementary information (ESI) available: Experimental procedures and characterization data. See DOI: 10.1039/c7cc02789c

Despite the diverse pharmacological activities of berberine, including anti-cancer and anti-inflammatory effects, the direct proteomic targets of berberine have remained largely unknown. Here, we have identified actin as a direct proteomic target of berberine using an affinity-based chemical probe....

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Main Authors: Yi, Chae-Min, Yu, Jihyun, Kim, Hyunbin, Lee, Na-Rae, Kim, Sang Won, Lee, Noh-Jin, Lee, Jun, Seong, Jihye, Kim, Nam-Jung, Inn, Kyung-Soo
Format: Article
Language:English
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Summary:Despite the diverse pharmacological activities of berberine, including anti-cancer and anti-inflammatory effects, the direct proteomic targets of berberine have remained largely unknown. Here, we have identified actin as a direct proteomic target of berberine using an affinity-based chemical probe. In addition, we found that actin assembly was significantly modulated by berberine in vitro at the biochemical level and cellular level. Actin was identified as a direct target of berberine via a chemoproteomic approach and its assembly was modulated by berberine.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc02789c