Bio-catalytic nanocompartments for in situ production of glucose-6-phosphateElectronic supplementary information (ESI) available: Experimental details and supporting results (TEM, DLS, SDS PAGE, additional fluorescence spectra). See DOI: 10.1039/c7cc04739h

• Main Authors: Lomora, M, Gunkel-Grabole, G, Mantri, S, Palivan, C. G
• Format: Article
• Language: English
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/c7cc04739h

Cells are sophisticated biocatalytic systems driving a complex network of biochemical reactions. A bioinspired strategy to create advanced functional systems is to design confined spaces for complex enzymatic reactions by using a combination of synthetic polymer assemblies and natural cell components. Here, we developed bio-catalytic nanocompartments that contain phosphoglucomutase protected by a biomimetic polymer membrane, which was permeabilized for reactants through insertion of an engineered α-hemolysin pore protein. These bio-catalytic nanocompartments serve for production of glucose-6-phosphate, and thus possess great potential for applications in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions. Bio-catalytic phosphoglucomutase based polymer nanocompartments permeabilized with engineered α-hemolysin for the production of glucose-6-phosphate needed to aid in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions.