A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cellsElectronic supplementary information (ESI) available: Synthesis of colloidal crystals, cancerous and normal cell culture, immunoassay procedure, scanning imaging and data analysis, supporting figures related to this article (Fig. S1-S7). See DOI: 10.1039/c7cc08167g

An ultra-high fluorescence enhancement for two dyes on photonic crystal films was achieved to construct a two-color immuno-dot blot assay. This assay was demonstrated to simultaneously detect chemokine receptor co-expressed in cancer cells and reveal their co-operative and subtle changes after bindi...

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Main Authors: Khan, Naseer Ullah, He, Hua, Wang, Xiaojuan, Ge, Baosheng, Wang, Qian, Liu, Xu, Lao, Jun, Wang, Yanan, Li, Jiqiang, Wang, Zhencai, Zhou, Shitan, Huang, Fang
Format: Article
Language:English
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Summary:An ultra-high fluorescence enhancement for two dyes on photonic crystal films was achieved to construct a two-color immuno-dot blot assay. This assay was demonstrated to simultaneously detect chemokine receptor co-expressed in cancer cells and reveal their co-operative and subtle changes after binding with respective ligands and drugs. A highly sensitive two-color dot-blot assay has been developed to simultaneously detect co-operative expression of chemokine receptors in cells.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc08167g