The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)Electronic supplementary information (ESI) available: HPLC data and NMR spectra. See DOI: 10.1039/c7gc01696d

By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M 3-5 , periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was...

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Main Authors: McKenna, S. M, Mines, P, Law, P, Kovacs-Schreiner, K, Birmingham, W. R, Turner, N. J, Leimkühler, S, Carnell, A. J
Format: Article
Language:English
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Summary:By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M 3-5 , periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO 2 hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values. One-pot multienzyme continuous cascade for the conversion of HMF to FDCA using HRP to activate GOaseM 3-5 in situ.
ISSN:1463-9262
1463-9270
DOI:10.1039/c7gc01696d