Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probesElectronic supplementary information (ESI) available. See DOI: 10.1039/c7ob03205f

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The l...

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Main Authors: Arai, Yohei, Yokoyama, Kouhei, Kawahara, Yuki, Feng, Qi, Ohta, Ippei, Shimoyama, Atsushi, Inuki, Shinsuke, Fukase, Koichi, Kabayama, Kazuya, Fujimoto, Yukari
Format: Article
Language:English
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Summary:As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules. Bacterial lipopeptide fluorescent probes were developed as TLR2 ligands, and their time-lapse monitoring of cellular internalization was performed.
ISSN:1477-0520
1477-0539
DOI:10.1039/c7ob03205f