A highly sensitive SERS probe for bisphenol A detection based on functionalized Au@Ag nanoparticles

Bisphenol A (BPA) usually exists in daily plastic products and its harmful effect on human health has been a popular topic. However, many conventional analytical methods need long sample preparation and measurement time, and some BPA biosensors suffer from the limitation of complex probe modificatio...

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Bibliographic Details
Published in:Analytical methods 2018-12, Vol.1 (47), p.5622-5628
Main Authors: Wang, Chun-Yang, Zeng, Yi, Shen, Ai-Guo, Hu, Ji-Ming
Format: Article
Language:English
Subjects:
Acids
Agglomeration
Analytical methods
Aptamers
Biosensors
Bisphenol A
Bottled water
Chitosan
Drinking water
Electrostatic properties
Environmental monitoring
Food safety
Gold
Nanoparticles
Noble metals
Nucleic acids
Phenols
Sample preparation
Silver
Substrates
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Summary:Bisphenol A (BPA) usually exists in daily plastic products and its harmful effect on human health has been a popular topic. However, many conventional analytical methods need long sample preparation and measurement time, and some BPA biosensors suffer from the limitation of complex probe modification. Herein, we designed a simple SERS-coded aptamer sensor for BPA detection based on the electrostatic interaction between chitosan encapsulated 4-MBA coated Au@Ag NPs and anti-BPA aptamers. The negatively charged nucleic acid anti-BPA aptamer will cause the aggregation of the positively charged noble metal nano-reinforced substrate in acid buffer systems, resulting in a significant enhancement of the Raman signal. The specific binding of BPA with nucleic acid anti-BPA aptamers reduced the degree of aggregation of nanoparticles and weakened the SERS signal. This SERS-coded aptamer sensor had a wide linear range of 0.01-1 ng mL −1 and the limit of detection (LOD) was as low as 2.8 pg mL −1 , and the method was successfully applied to detect BPA in bottled water within 15 min. As a rapid, simple, ultrasensitive and highly selective analytical technique for the detection of BPA, this SERS-based method would be important in biomedical diagnostics, environmental monitoring and food safety. The presence of BPA reduced the orange-to-grey color change (aggregation degree) of the SERS probe and weakened the enhancement of the Raman signal.
ISSN:1759-9660
1759-9679
DOI:10.1039/c8ay01966e