In vitro selection of electrochemical peptide probes using bioorthogonal tRNA for influenza virus detectionElectronic supplementary information (ESI) available: Preparation of tRNA, non-natural amino acid, peptides. Interaction and specificity analysis by fluorescence and electropolymerization. See DOI: 10.1039/c8cc01775a

An electrosensitive peptide probe has been developed from an in vitro selection technique using biorthogonal tRNA prepared with an electroreactive non-natural amino acid, 3,4-ethylenedioxythiophene-conjugated aminophenylalanine. The selected probe quantitatively detected the influenza virus based on...

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Main Authors: , Tada, Seiichi, Zhu, Liping, Uzawa, Takanori, Minagawa, Noriko, Luo, Shyh-Chyang, Zhao, Haichao, Yu, Hsiao-hua, Aigaki, Toshiro, Ito, Yoshihiro
Format: Article
Language:English
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Summary:An electrosensitive peptide probe has been developed from an in vitro selection technique using biorthogonal tRNA prepared with an electroreactive non-natural amino acid, 3,4-ethylenedioxythiophene-conjugated aminophenylalanine. The selected probe quantitatively detected the influenza virus based on a signal "turn-on" mechanism. The developed strategy could be used to develop electrochemical biosensors toward a variety of targets. An electro-polymerizable peptide probe containing 3,4-ethylenedioxythiophene-conjugated aminophenylalanine was developed for "turn-on" detection of influenza virus.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc01775a