3-Selenocyanate-indoles as new agents for the treatment of superficial and mucocutaneous infectionsElectronic supplementary information (ESI) available: Synthesis of 3-selenocyanate-indoles 4, tables of the mechanism of action, antibacterial susceptibility test, and HET-CAM assay. See DOI: 10.1039/c8nj04935a

• Main Authors: Quatrin, Priscilla Maciel, Dalla Lana, Daiane Flores, Bazana, Luana Candice Genz, de Oliveira, Luis Flávio Souza, Lettieri Teixeira, Mario, Silva, Edilma Elaine, Lopes, William, Canto, Rômulo Faria Santos, Silveira, Gustavo Pozza, Fuentefria, Alexandre Meneghello
• Format: Article
• Language: English
• ISSN: 1144-0546; 1369-9261
• DOI: 10.1039/c8nj04935a

The development of resistance to the current antifungal agents is an alarming problem. Therefore, the search for new molecules capable of combating fungi infections is imperative. This study presents the in vitro activities of a library of seven 3-selenocyanate-indoles against Candida spp. and dermatophytes of the genera Trichophyton and Microsporum . The antifungal susceptibility of compounds 4a and 4b presented geometric mean values of 4.1 and 6.0 μg mL −1 against Candida spp. and 1.2 and 2.2 μg mL −1 against dermatophytes following the CLSI guidelines. The 3-selenocyanate-indole 4a showed a fungicidal effect against the whole fungal panel. The toxicological results revealed that the selenocyanates 4a and 4b did not show mutagenicity or cause changes in the human leukocyte cells and were classified as non-irritant by the ex vivo HET-CAM test. The mechanism of action of the 3-selenocyanate-indoles has not been clearly elucidated. However, a genotoxic potential in higher concentrations, observed by the comet assay, leads us to believe that these molecules have their mechanism of action related to the nucleus of the fungal cells. The development of resistance to the current antifungal agents is an alarming problem.