Au/FeO core-shell nanoparticles are an efficient immunochromatography test strip performance enhancer-a comparative study with Au and FeO nanoparticles

• Published in: RSC advances 2018-04, Vol.8 (25), p.1464-1471
• Main Authors: Huang, Jiaoling, Xie, Zhixun, Xie, Liji, Xie, Zhiqin, Luo, Sisi, Deng, Xianwen, Huang, Li, Zeng, Tingting, Zhang, Yanfang, Wang, Sheng, Zhang, Minxiu
• Format: Article
• ISSN: 2046-2069
• DOI: 10.1039/c8ra00185e

Immunochromatography test strips that use metal particles constructed from Au, Fe 3 O 4 , and Au/Fe 3 O 4 nanoparticles were developed for the rapid detection of avian influenza virus subtype H7 (AIV H7). The principle of this immunochromatography test strip was based on a sandwich immunoreaction in which AIV H7 antigens bind specifically to their corresponding antibodies on a nitrocellulose membrane. An antibody-metal (Au, Fe 3 O 4 or Au/Fe 3 O 4 ) nanoparticle conjugate was used as a label and coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and a control zone, an anti-H7 polyclonal antibody and an anti-IgG antibody were immobilized onto the nitrocellulose membrane, respectively. Positive samples displayed brown/red lines in the test and control zones of the nitrocellulose membrane, whereas negative samples resulted in a brown/red line only in the control zone. The limit of detection (LOD) of the Au/Fe 3 O 4 nanoparticle-based immunochromatography test strips was found to be 10 3.5 EID 50 (EID 50 : 50% Egg Infective Dose), which could be visually detected by the naked eye within 15 min. In addition, 200 clinical samples were tested using the Au/Fe 3 O 4 nanoparticle-based immunochromatography test strip to estimate its performance, and seven were positive for AIV H7. In summary, the Au/Fe 3 O 4 nanoparticle-based immunochromatography test strip offers a simple and cost-effective tool for the rapid detection of AIV H7. Immunochromatography test strips that use metal particles constructed from Au, Fe 3 O 4 , and Au/Fe 3 O 4 nanoparticles were developed for the rapid detection of avian influenza virus subtype H7 (AIV H7).