Synthesis of glycopolymers with specificity for bacterial strains bacteria-guided polymerization

Identifying probiotics and pathogens is of great interest to the health of the human body. It is critical to develop microbiota-targeted therapies to have high specificity including strain specificity. In this study, we have utilized E. coli MG1655 bacteria as living templates to synthesize glycopol...

Full description

Saved in:
Bibliographic Details
Published in:Chemical science (Cambridge) 2019-05, Vol.1 (2), p.5251-5257
Main Authors: Luo, Yan, Gu, Yan, Feng, Ruyan, Brash, John, Eissa, Ahmed M, Haddleton, David M, Chen, Gaojian, Chen, Hong
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Identifying probiotics and pathogens is of great interest to the health of the human body. It is critical to develop microbiota-targeted therapies to have high specificity including strain specificity. In this study, we have utilized E. coli MG1655 bacteria as living templates to synthesize glycopolymers in situ with high selectivity. By this bacteria-sugar monomer-aptation-polymerization (BS-MAP) method, we have obtained glycopolymers from the surface of bacteria which can recognize template bacteria from two strains of E. coli and the specific bacteria-binding ability of glycopolymers was confirmed by both bacterial aggregation experiment and QCM-D measurements. Furthermore, the synthesized glycopolymers have shown a powerful inhibitory ability which can prevent bacteria from harming cells in both anti-infection and co-culture tests. Glycopolymers with specificity to template strain of E. coli were synthesised by the bacteria-sugar monomer-aptation-polymerization.
ISSN:2041-6520
2041-6539
DOI:10.1039/c8sc05561k