The preparation of bifunctional hybrid nano-flowers and their application in the enzyme-linked immunosorbent assay for detection

• Published in: Analyst (London) 2021-01, Vol.146 (1), p.338-347
• Main Authors: Wang, Tiantian, Li, Xiangguang, Chen, Lili, Zhang, Youhuan, Zheng, Yujun, Yu, Linjin, Ye, Zhiyu, Wang, Huaqian, Cui, Xiping, Zhao, Suqing
• Format: Article
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d0an01533d

As the infection by Helicobacter pylori ( H. pylori , HP) remains for a lifetime and may induce diseases such as gastric cancer, it is vital to detect and diagnose it. A new non-invasive indirect enzyme-linked immunosorbent assay (iELISA) method based on nano-flowers (NFs) is very advantageous for the sensitive detection of HP. Furthermore, the established iELISA method based on the organic-inorganic bifunctional hybrid nano-flowers including rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab-HRP@Cu 2+ NFs) showed linearity with HP at a concentration of 0-10 5 CFU mL −1 ( R 2 = 0.9997). Moreover, the limit of detection (LOD) reached 50 CFU mL −1 , and not only was the detection sensitivity 20 times higher than that based on rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab-HRP) but also the stability of R-HP-Ab-HRP in NFs was improved. In addition, the OD 450 nm value was still linearly related to the concentration of HP at a range of 0-10 5 CFU mL −1 ( R 2 = 0.9952) with a LOD of 50 CFU mL −1 in an artificial saliva system. This study provided a sensitive, low-cost and convenient method for the non-invasive detection of HP. As the infection by Helicobacter pylori ( H. pylori , HP) remains for a lifetime and may induce diseases such as gastric cancer, it is vital to detect and diagnose it.