dsDNA-templated fluorescent copper nanoparticles for the detection of lipopolysaccharides

The introduction of lipopolysaccharides (LPS) or endotoxins that originate from Gram-negative bacteria into the human blood stream induces a severe immune response that can lead to septic shock, and even death. Hence, the accurate detection of LPS is of great importance in the medical and pharmaceut...

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Bibliographic Details
Published in:Analytical methods 2021-01, Vol.13 (2), p.186-191
Main Authors: , Gorthi, Sai Siva
Format: Article
Language:English
Subjects:
Aptamers
Assaying
Bacteria
Copper
DNA
Endotoxins
Fluorescence
Fluorescent Dyes
Gram-negative bacteria
Humans
Immune response
Lipopolysaccharides
Metal Nanoparticles
Nanoparticles
Reagents
Room temperature
Septic shock
Temperature requirements
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Summary:The introduction of lipopolysaccharides (LPS) or endotoxins that originate from Gram-negative bacteria into the human blood stream induces a severe immune response that can lead to septic shock, and even death. Hence, the accurate detection of LPS is of great importance in the medical and pharmaceutical sectors. This paper proposes a novel label-free fluorescence assay for the detection of LPS utilizing aptamers and the interference synthesis of dsDNA-templated copper nanoparticles. The assay can be performed at room temperature and does not require expensive reagents. The proposed assay has a limit of detection of 0.95 ng ml −1 of LPS, and the fluorescence emission from the copper nanoparticles was found to vary linearly with the concentration of LPS over a wide range (1 to 10 5 ng ml −1 ) with R 2 = 0.9877. A label-free fluorescence assay utilizing aptamers and the interference synthesis of dsDNA-templated copper nanoparticles is proposed for the detection of lipopolysaccharides.
ISSN:1759-9660
1759-9679
DOI:10.1039/d0ay01906b