O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first 17 O NMR spectroscopy study of isotopically enriched cho...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2020-11, Vol.56 (92), p.14499-1452
Main Authors: Rowlands, Lucy J, Marks, Adam, Sanderson, John M, Law, Robert V
Format: Article
Language:English
Online Access:Get full text
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Summary:Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first 17 O NMR spectroscopy study of isotopically enriched cholesterol. We demonstrate the 17 O chemical shift is dependent on hydrogen bonding, providing a novel method for the study of cholesterol in bilayers. Solid state NMR of 17 O enriched cholesterol in bilayers reveals the chemical shift depends on extent of hydrogen bonding.
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc05466f