Cyclopentane FIT-PNAs: bright RNA sensors

Cyclopentane modified FIT-PNA (cpFIT-PNA) probes are reported as highly emissive RNA sensors with the highest reported brightness for FIT-PNAs. Compared to FIT-PNAs, cpFIT-PNAs have improved mismatch discrimination for several pyrimidine-pyrimidine single nucleotide variants (SNVs). Cyclopentane-mod...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-01, Vol.57 (4), p.54-543
Main Authors: Tepper, Odelia, Zheng, Hongchao, Appella, Daniel H, Yavin, Eylon
Format: Article
Language:English
Subjects:
Benzothiazoles - chemistry
Cyclopentanes - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Limit of Detection
Nucleic Acid Hybridization
Nucleotides
Peptide Nucleic Acids - chemistry
Peptide Nucleic Acids - genetics
Quinolines - chemistry
RNA - analysis
RNA - genetics
Sensors
Spectrometry, Fluorescence
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Summary:Cyclopentane modified FIT-PNA (cpFIT-PNA) probes are reported as highly emissive RNA sensors with the highest reported brightness for FIT-PNAs. Compared to FIT-PNAs, cpFIT-PNAs have improved mismatch discrimination for several pyrimidine-pyrimidine single nucleotide variants (SNVs). Cyclopentane-modified PNA monomers surrounding BisQ (surrogate base) significantly increase the sensitivity and selectivity for RNA detection by FIT-PNAs.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d0cc07400d