Fabrication of 3D concentric amphiphilic microparticles to form uniform nanoliter reaction volumes for amplified affinity assays

Reactions performed in uniform microscale volumes have enabled numerous applications in the analysis of rare entities ( e.g. cells and molecules). Here, highly monodisperse aqueous droplets are formed by simply mixing microscale multi-material particles, consisting of concentric hydrophobic outer an...

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Bibliographic Details
Published in:Lab on a chip 2020-10, Vol.2 (19), p.353-3514
Main Authors: Destgeer, Ghulam, Ouyang, Mengxing, Wu, Chueh-Yu, Di Carlo, Dino
Format: Article
Language:English
Subjects:
Amplification
Crosstalk
Immunoassay
Microfluidics
Microparticles
Multiplexing
Nozzle design
Prepolymers
Three dimensional printing
Ultraviolet radiation
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Summary:Reactions performed in uniform microscale volumes have enabled numerous applications in the analysis of rare entities ( e.g. cells and molecules). Here, highly monodisperse aqueous droplets are formed by simply mixing microscale multi-material particles, consisting of concentric hydrophobic outer and hydrophilic inner layers, with oil and water. The particles are manufactured in batch using a 3D printed device to co-flow four concentric streams of polymer precursors which are polymerized with UV light. The cross-sectional shapes of the particles are altered by microfluidic nozzle design in the 3D printed device. Once a particle encapsulates an aqueous volume, each "dropicle" provides uniform compartmentalization and customizable shape-coding for each sample volume to enable multiplexing of uniform reactions in a scalable manner. We implement an enzymatically-amplified immunoassay using the dropicle system, yielding a detection limit of
ISSN:1473-0197
1473-0189
DOI:10.1039/d0lc00698j