Self-assembly synthesis of Ag@PANI nanocomposites as a tandem enzyme utilizing a highly efficient label-free SERS method to detect saccharides

Surface Enhanced Raman Scattering (SERS), with good reproducibility and stability, is an analytical testing technique that uses metals or metal nanoparticles as detection substrates, and it can also be used to characterize the information from molecular vibration. In this paper, we first report a fa...

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Bibliographic Details
Published in:New journal of chemistry 2020-10, Vol.44 (38), p.16384-16389
Main Authors: Tang, Ru-Yi, Lei, Zhang, Weng, Yi-Jin, Xia, Xue-Min, Zhang, Xia
Format: Article
Language:English
Subjects:
Carbohydrates
Enzymes
Hydrogen peroxide
Lactose
Nanocomposites
Nanoparticles
Oxidation
Peroxidase
Polyanilines
Raman spectra
Self-assembly
Silver
Stability analysis
Substrates
Sucrose
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Summary:Surface Enhanced Raman Scattering (SERS), with good reproducibility and stability, is an analytical testing technique that uses metals or metal nanoparticles as detection substrates, and it can also be used to characterize the information from molecular vibration. In this paper, we first report a facile one-step approach that allows the fabrication of assembled Ag NPs induced by polyaniline (PANI). Ag@PANI nanocomposites have the dual functions of saccharide-oxidase-like and peroxidase-like activities. Therefore, they can be used as a tandem enzyme to produce hydrogen peroxide from saccharides and catalyze the oxidation of TMB. At the same time, they can be considered as a SERS substrate to obtain the characteristic peaks of TMB ox . In addition, on the basis of SERS, the effects of the Ag + concentration, temperature and pH on the activity of Ag@PANI nanocomposites as the tandem enzyme are further explored. Under the optimal conditions, the common saccharides (sucrose, fructose, glucose and lactose) were detected with linear correlation ( R 2 ) in different concentration ranges, and ultra-sensitive detection of various saccharides under label-free conditions was achieved. Surface Enhanced Raman Scattering (SERS), with good reproducibility and stability, is an analytical testing technique that uses metals or metal nanoparticles as detection substrates, and it can also be used to characterize the information from molecular vibration.
ISSN:1144-0546
1369-9261
DOI:10.1039/d0nj02073g