Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf signalling platform

We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout. The so-called DSN-PGM sensing platform is sensitive, specific and general to both microRNA and long virus RNA. The detection procedur...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-06, Vol.57 (46), p.5714-5717
Main Authors: Guo, Lulu, Li, Huan, Zhao, Rujian, Tang, Yidan, Li, Bingling
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
Duplex plating
Nuclease
Ribonucleic acid
RNA
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Summary:We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout. The so-called DSN-PGM sensing platform is sensitive, specific and general to both microRNA and long virus RNA. The detection procedure is simple and instrument-free, thus holding particular potential in the development of portable and point-of-care measurements. We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc01715b