A universal rolling circle amplification for label-free and highly specific nucleic acid sensing

Traditional RCA methods face some drawbacks including limited specificity and amplification templates with sequence dependence. Herein, a universal RCA (URCA) strategy for label-free nucleic acid sensing with high specificity was proposed, which could be used for sensing of different nucleic acids w...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2022-10, Vol.58 (84), p.11863-11866
Main Authors: Sun, Mengxu, Liu, Birong, Li, Tong, Li, Chunrong, Duan, Wen-Jun, Xie, Baoping, Li, Min-Min, Chen, Jin-Xiang, Dai, Zong, Chen, Jun
Format: Article
Language:English
Subjects:
Amplification
Nucleic acids
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Summary:Traditional RCA methods face some drawbacks including limited specificity and amplification templates with sequence dependence. Herein, a universal RCA (URCA) strategy for label-free nucleic acid sensing with high specificity was proposed, which could be used for sensing of different nucleic acids without redesigning or synthesizing new amplification templates. The URCA strategy also showed high accuracy for miRNA analysis in practical samples. A universal rolling circle amplification was proposed for label-free and highly specific nucleic acid sensing.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc04058a