growth of metal-organic frameworks in nanochannels for highly sensitive microcystin-LR detection

• Published in: Environmental science. Nano 2023-03, Vol.1 (3), p.834-842
• Main Authors: Zhang, Wei-Qi, Tu, Yi-Dan, Wang, Xu-Gang, Huang, Yu, Xia, Fan
• Format: Article
• ISSN: 2051-8153; 2051-8161
• DOI: 10.1039/d2en01119k

Microcystin-LR (MC-LR) is a known hepatotoxin that is the most abundant and potent cyanobacterial toxin. The development of a strategy for sensitive MC-LR detection is highly demanded. In this work, a novel solid-state nanochannel-based sensing system was constructed by in situ growth of metal-organic frameworks (MOFs) on anodic aluminum oxide (AAO) nanochannels and modification with an MC-LR-specific aptamer as a probe. The MOF was employed to reduce the effective diameter of the nanochannels and provided abundant functional active sites for the probes. As a result, highly sensitive MC-LR detection was realized with the limit of detection reaching 0.004 ng mL −1 . Compared to the nanochannels without MOF modification, the sensitivity has been significantly improved, by around 10 000-fold. Furthermore, highly reliable detection of MC-LR in various water samples was also achieved, with a relative standard deviation of less than 5.46% and recovery between 93.90% and 106.73%. This strategy provides significant insight for practical applications of nanochannel-based sensing systems, including environmental contaminant detection, food analysis, and medical analysis. Harmful algal blooms lead to cyanobacteria toxins increase in natural water. A novel nanochannel-based sensing system utilizing metal-organic frameworks and DNA aptamers was developed for highly sensitive detecting microcystin-LR in real water.