Rapid electrochemical detection of MiRNA-21 facilitated by the excellent catalytic ability of Pt@CeO nanospheres

• Published in: RSC advances 2022-04, Vol.12 (19), p.11867-11876
• Main Authors: Chen, Peiwu, Jiang, Lan, Xie, Xianjin, Sun, Dong, Liu, Jinyao, Zhao, Yuefeng, Li, Yuhao, Balbín Tamayo, Abel Ibrahim, Liu, Baolin, Miao, Yuqing, Ouyang, Ruizhuo
• Format: Article
• ISSN: 2046-2069
• DOI: 10.1039/d2ra01047j

Pt@CeO 2 nanospheres (NSs) were first synthesized by simply mixing Ce(NO 3 ) 3 and K 2 PtCl 4 under the protection of pure argon at 70 °C for 1 h, which exhibited excellent catalytic ability toward hydrogen peroxide (H 2 O 2 ). An electrochemical biosensor was successfully developed using Pt@CeO 2 NSs as a capture probe for the ultra-sensitive and fast detection of miRNA-21, a new type of biomarker for disease diagnostics, especially for cancer. During the step-by-step construction process of the RNA sensor, Pt@CeO 2 NSs were functionalized with streptavidin (SA) to obtain SA-Pt@CeO 2 NSs through amide bonds. Gold nanoparticles (Au NPs) were electrodeposited on the surface of the glassy carbon electrode to improve the transmission capacity of electrons and provided Au atoms for fixing the thiolated capture probe (SH-CP) with a hairpin structure on the electrode via forming Au-S bonds. The target miRNA-21 specifically hybridized with SH-CP and opened the hairpin structure to form a rigid duplex so as to activate the biotin at the end of the capture probe. SA-Pt@CeO 2 NSs were thus specially attached to the electrode surface through the biotin-streptavidin affinity interaction, finally leading to the significant signal amplification. The ultra-sensitive and rapid detection of miRNA-21 was finally realized as expected benefiting from the excellent catalytic ability of Pt@CeO 2 NSs toward H 2 O 2 in a wide linear concentration range from 10 fM to 1 nM with the detection limit as low as 1.41 fM. The results achieved with this new RNA sensor were quite satisfactory during the blood sample analysis. New Pt@CeO 2 nanospheres with advanced catalytic ability were successfully applied to fabricate a highly sensitive electrochemical RNA sensor for rapid detection of miRNA-21 in real blood samples.