NaBiF upconversion nanoparticle-based electrochemiluminescent biosensor for O157 : H7 detection

• Published in: RSC advances 2022-10, Vol.12 (46), p.3174-318
• Main Authors: Liu, Danqing, Lv, Xingxing, Zhao, Chaoyue, Li, Jiayue, Huang, Jinmei, Weng, Ling, He, Liangcan, Liu, Shaoqin
• Format: Article
• ISSN: 2046-2069
• DOI: 10.1039/d2ra05217b

Foodborne or water-borne pathogens pose great threats to human beings and animals. There is an urgent need to detect pathogens with cheap, rapid and sensitive point-of-care diagnostic assays. Herein, we report the electrochemiluminescent (ECL) behaviors of NaBiF 4  : Yb 3+ /Er 3+ upconversion nanoparticles (UCNPs) which were synthesized via a fast and environment-friendly method at room temperature for the first time. The UCNPs together with K 2 S 2 O 8 exhibit high ECL intensity and stable cathodic signals. Further, the Au nanoparticles (Au NPs) and Anti- E. coli O157 : H7 antibody were assembled on the surface of UCNPs successively to construct a novel ECL immunosensor for the detection of deadly E. coli O157 : H7. The as-prepared ECL immunosensor reveals high sensitivity to E. coli O157 : H7 in a linear range of 200-100 000 CFU mL −1 , and the minimum detection limit could reach up to 138 CFU mL −1 . The designed UCNP-based biosensor demonstrates high specificity, good stability and remarkable repeatability, and the strategy will provide a sensitive and selective method for rapid detection of E. coli O157 : H7 in food safety and preclinical diagnosis. The ECL behaviors of NaBiF 4  : Yb 3+ /Er 3+ UCNPs synthesized via a fast and environment-friendly method are reported for the first time. UCNPs-based ECL biosensor shows a wide detection range with low detection limit of 138 CFU mL −1 for E. coli O157 : H7.