Electrochemiluminescence immunoassay strategies based on a hexagonal Ru-MOF and MoS@GO nanosheets: detection of 5-fluorouracil in serum samples

• Published in: Analyst (London) 2023-04, Vol.148 (8), p.1694-172
• Main Authors: Ma, Guoyu, Peng, Lu, Zhang, SunXiaoYi, Wu, Kang, Deng, Anping, Li, Jianguo
• Format: Article
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d3an00190c

Herein, a competitive-type electrochemiluminescence immunosensor for ultrasensitive detection of 5-fluorouracil (5-FU) was fabricated. Ruthenium( ii )-metal-organic framework (Ru-MOF) nanosheets were selected to act a promising ECL luminophore using tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium( ii ) dichloride (Ru(dcbpy) 3 2+ ) as the organic ligand. The two-dimensional (2D) Ru-MOF nanosheets achieved an increased loading of Ru(dcbpy) 3 2+ and effectively prevented leakage of the ECL emitter during application, which exhibited satisfactory ECL performance. Thin two-dimensional MoS 2 @GO was used to modify the electrode as the sensing platform for improving the electron transfer rate and loading more 5-FU coating antigens due to its large specific surface area and piezoelectric catalytic efficiency. Under the optimized conditions, the proposed immunosensor presented high sensitivity, a wide detection range (0.0001 ng-100 ng mL −1 ), a low limit of detection (0.031 pg mL −1 , S/N = 3), good specificity and stability. Furthermore, the immunosensor was successfully applied for the detection of 5-FU in human serum samples with satisfactory results, proving this strategy has potential applications in bioanalysis and clinical diagnosis. Herein, a competitive-type electrochemiluminescence immunosensor for ultrasensitive detection of 5-fluorouracil (5-FU) was fabricated.