Excitonic coupling of RNA-templated merocyanine dimer studied by higher-order transient absorption spectroscopy

We report the synthesis and spectroscopic analysis of RNA containing the barbituric acid merocyanine rBAM2 as a nucleobase surrogate. Incorporation into RNA strands by solid-phase synthesis leads to fluorescence enhancement compared to the free chromophore. In addition, linear absorption studies sho...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2023-06, Vol.59 (48), p.7395-7398
Main Authors: Dietzsch, Julia, Jayachandran, Ajay, Mueller, Stefan, Höbartner, Claudia, Brixner, Tobias
Format: Article
Language:English
Subjects:
Absorption spectroscopy
Chromophores
Dimers
Excitons
Fluorescence
Solid phase synthesis
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Summary:We report the synthesis and spectroscopic analysis of RNA containing the barbituric acid merocyanine rBAM2 as a nucleobase surrogate. Incorporation into RNA strands by solid-phase synthesis leads to fluorescence enhancement compared to the free chromophore. In addition, linear absorption studies show the formation of an excitonically coupled H-type dimer in the hybridized duplex. Ultrafast third- and fifth-order transient absorption spectroscopy of this non-fluorescent dimer suggests immediate (sub-200 fs) exciton transfer and annihilation due to the proximity of the rBAM2 units. An RNA duplex modified with a merocyanine dimer was synthesized and exciton dynamics within this dimer were studied using ultrafast third- and fifth-order transient absorption spectroscopy.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc02024j