A facile method for purifying DNA-modified small particles and soft materials using aqueous two-phase systems

We herein report a convenient and effective method for the purification of DNA-conjugated materials with a benchtop minicentrifuge. We demonstrate the fast isolation of DNA-modified small gold nanoparticles (5 nm), liposomes, and DNA nanostructures using fluorescent methods and gel electrophoresis....

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2023-07, Vol.59 (59), p.913-9133
Main Authors: Duan, Xiaoman, Liu, Biwu
Format: Article
Language:English
Subjects:
Binary systems
DNA - chemistry
Electrophoresis
Fluorescence
Gold - chemistry
Metal Nanoparticles - chemistry
Nanoparticles
Nanostructures - chemistry
Nanotechnology - methods
System effectiveness
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Summary:We herein report a convenient and effective method for the purification of DNA-conjugated materials with a benchtop minicentrifuge. We demonstrate the fast isolation of DNA-modified small gold nanoparticles (5 nm), liposomes, and DNA nanostructures using fluorescent methods and gel electrophoresis. Our method is cost-effective and efficient and would accelerate the development of DNA nanotechnology. The aqueous two-phase system is used to facilitate the purification of DNA-conjugated nanomaterials simply with a benchtop minicentrifuge.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc02350h