An allosteric palindromic hairpin probe based dual-mode interactive strand displacement amplification enables robust miRNA biosensing

This study introduces an allosteric palindromic hairpin probe (APHP)-based dual-mode interactive strand displacement amplification (DMI-SDA) system for ultrasensitive detection of microRNA-155. The system achieves exceptional signal amplification and improved signal preservation using dimeric G-trip...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-03, Vol.6 (21), p.291-2913
Main Authors: Wang, Qi, Zhou, Tong, Xue, Danni, Yang, Haidong, Sui, Zhuqi, Yuan, Xinyue, Xu, Jianguo
Format: Article
Language:English
Subjects:
Amplification
Ribonucleic acid
RNA
Robustness
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Summary:This study introduces an allosteric palindromic hairpin probe (APHP)-based dual-mode interactive strand displacement amplification (DMI-SDA) system for ultrasensitive detection of microRNA-155. The system achieves exceptional signal amplification and improved signal preservation using dimeric G-triplexes as signal reporters, enabling robust detection of miRNA-155, representing a promising avenue in molecular diagnosis. An allosteric palindromic hairpin probe enables a dimer-G-triplex-productive dual-mode interactive strand displacement amplification in miRNA biosensing with improved signal preservation and response.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc06265a