Antimicrobial activity of safe concentrations of ozone, hydrogen peroxide, and triethylene glycol in air and surfaces

• Published in: Environmental science: atmospheres 2024-06, Vol.4 (6), p.62-633
• Main Authors: Truyols-Vives, Joan, Botella-Grau, Salut, Mercader-Barceló, Josep, Baldoví, Herme G
• Format: Article
• Language: English
• ISSN: 2634-3606; 2634-3606
• DOI: 10.1039/d3ea00156c

Monitoring and control of indoor air hygiene has gained much interest since the COVID-19 pandemic because the airborne route is the main pathway for the spread of SARS-CoV-2 and other pathogens, making it necessary to develop strategies to mitigate airborne transmission of diseases. This work addresses indoor breathable air hygiene by proposing the " in situ " reduction of airborne microorganisms with the nebulization of low and safe concentrations of hydrogen peroxide (H 2 O 2, 0.5 and 1 ppm), ozone (O 3, 0.06 and 0.2 ppm), triethylene glycol (TEG, 17.1, 52 and 171.2 ppm), and their combinations. The antimicrobial activity was evaluated in an office room by assessing the viability of commercial extremophile sporulated bacteria and naturally present bacteria and fungi in surfaces and air. All three chemicals individually dispersed reduced the viability of sporulated bacteria and naturally occurring microorganisms. Binary combinations were more effective than individual agents in the case of the H 2 O 2 and O 3 mixture against sporulated bacteria, and the O 3 and TEG mixture against airborne and surface bacteria. The ternary mixture was the most effective against commercial sporulated bacteria and airborne microorganisms. These results illustrate that the application of low and safe concentrations of antimicrobial compounds in indoor air could be an interesting strategy to reduce infection risk. The application of low and non-toxic concentrations of antimicrobial compounds in indoor air is an interesting strategy to reduce airborne bacteria and fungi thereby reducing infection risk.