Cytotoxic cytochalasans from cultures of the fungus TBRC-BCC 79240

• Published in: RSC advances 2023-04, Vol.13 (16), p.1564-1576
• Main Authors: Kornsakulkarn, Jittra, Auncharoen, Patchanee, Khonsanit, Artit, Boonyuen, Nattawut, Thongpanchang, Chawanee
• Format: Article
• ISSN: 2046-2069
• DOI: 10.1039/d3ra00042g

Fourteen new cytochalasans, brunnesins A-N ( 1-14 ), along with eleven known compounds, were isolated from the culture extracts of the insect pathogenic fungus Metarhizium brunneum strain TBRC-BCC 79240. The compound structures were established by spectroscopy, X-ray diffraction analysis, and electronic circular dichroism. Compound 4 exhibited antiproliferative activity against all cell lines tested (mammalian), with 50% inhibition concentration (IC 50 ) values ranging from 2.09 to 16.8 μg mL −1 . Compounds 6 and 16 were shown to be bioactive only against non-cancerous Vero cells (IC 50 4.03 and 0.637 μg mL −1 , respectively) whereas compounds 9 and 12 were bioactive only against NCI-H187 small-cell lung cancer cells (IC 50 18.59 and 18.54 μg mL −1 , respectively). Compounds 7 , 13 , and 14 showed cytotoxicity against NCI-H187 and Vero cell lines with IC 50 values ranging from 3.98-44.81 μg mL −1 . Fourteen new cytochalasans, brunnesins A-N ( 1-14 ), along with eleven known compounds, were isolated from the culture extracts of the insect pathogenic fungus Metarhizium brunneum strain TBRC-BCC 79240.