A rhodamine based near-infrared fluorescent probe for selective detection of Cu ions and its applications in bioimaging

• Published in: Analytical methods 2024-12, Vol.17 (1), p.178-183
• Main Authors: Chang, Zhijian, Li, Shumeng, Ye, Jia-Hai, Lin, Fuyan, Chen, Yuncong, Guo, Zijian, Gong, Xuedong
• Format: Article
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/d4ay01922a

A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu 2+ in EtOH/H 2 O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0-50 μM). Detection of Cu 2+ is undisturbed at physiological pH levels of 5-9. This recognition mechanism is attributed to the formation of a 1 : 1 complex between TM2 and Cu 2+ , validated by Job's plot, 1 H NMR titration, and LC-MS experiments. Moreover, the successful fluorescence imaging of Cu 2+ both in vitro and in vivo was also accomplished. A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm).