VPg-based bidirectional synthetic mRNA circuits enable orthogonal protein regulation for high-resolution cell separation

Synthetic mRNA circuits commonly sense input to produce binary output signals for cell separation. Based on virus-origin cap-independent translation initiation machinery and RBP-aptamer interaction, we designed smart synthetic mRNA-based circuits that sense single input molecules to bidirectionally...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-05, Vol.6 (41), p.5427-543
Main Authors: Liang, Zhenghua, Hu, Yaxin, Li, Cheuk Yin, Yau, Wai Laam, Tan, Kaixin, Kuang, Yi
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Cell Separation - methods
Circuit design
High resolution
Humans
Resolution cell
RNA, Messenger - genetics
RNA, Messenger - metabolism
Separation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Synthetic mRNA circuits commonly sense input to produce binary output signals for cell separation. Based on virus-origin cap-independent translation initiation machinery and RBP-aptamer interaction, we designed smart synthetic mRNA-based circuits that sense single input molecules to bidirectionally tune output signals in an orthogonal manner, enabling high-resolution separation of cell populations. Synthetic mRNA-based circuits for high-resolution cell separation: a simple and effective bidirectional regulation strategy.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d4cc01725k