Aptamer-assisted phage display: enhancing checkpoint inhibition with a peptide and an aptamer targeting distinct sites on a single PD-L1 protein

Utilizing a novel approach known as aptamer-assisted phage display (APD), we identified an anti-PD-L1 peptide, NV Pep, capable of simultaneous binding to PD-L1 alongside the DNA aptamer MJ5C. Combined inhibition using NV Pep and MJ5C demonstrated significant enhancement compared to individual ligand...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-07, Vol.6 (59), p.757-7573
Main Authors: Arya, Satya Prakash, Thennakoon, Siddhartha Kalpa Samadhi, Phuoc, Chien Minh Tran, Silwal, Achut Prasad, Jahan, Raunak, Postema, Rick Mason, Timilsina, Hari, Reynolds, Andrew Michael, Tan, Xiaohong
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Aptamers, Nucleotide - pharmacology
B7-H1 Antigen - antagonists & inhibitors
B7-H1 Antigen - metabolism
Humans
Immune Checkpoint Inhibitors - chemistry
Immune Checkpoint Inhibitors - pharmacology
Peptide Library
Peptides - chemistry
Peptides - metabolism
Peptides - pharmacology
Phages
Programmed Cell Death 1 Receptor - antagonists & inhibitors
Programmed Cell Death 1 Receptor - metabolism
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Summary:Utilizing a novel approach known as aptamer-assisted phage display (APD), we identified an anti-PD-L1 peptide, NV Pep, capable of simultaneous binding to PD-L1 alongside the DNA aptamer MJ5C. Combined inhibition using NV Pep and MJ5C demonstrated significant enhancement compared to individual ligands against the PD-1/PD-L1 interaction. Utilizing a novel approach known as aptamer-assisted phage display (APD), we identified an anti-PD-L1 peptide, NV Pep, capable of simultaneous binding to PD-L1 alongside the DNA aptamer MJ5C.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d4cc02132k