Modifications of Cellular Autofluorescence Emission Spectra under Oxidative Stress Induced by 1 α,25dihydroxyvitamin D3 and its Analog EB1089

We attempted to characterize the cellular autofluorescence phenomenon of living HL-60 cells and to appraise its modifications under oxidative stress conditions induced by 1α,25(OH)2D3 (VD3) and its analog EB1089. Autofluorescence emission spectra of human promyelocytic HL-60 leukemic cells were moni...

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Bibliographic Details
Published in:Technology in cancer research & treatment 2004-08, Vol.3 (4), p.383-391
Main Authors: Bondza-Kibangou, Patrick, Millot, Christine, Dufer, Jean, Millot, Jean-Marc
Format: Article
Language:English
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Summary:We attempted to characterize the cellular autofluorescence phenomenon of living HL-60 cells and to appraise its modifications under oxidative stress conditions induced by 1α,25(OH)2D3 (VD3) and its analog EB1089. Autofluorescence emission spectra of human promyelocytic HL-60 leukemic cells were monitored using laser scanning confocal microspectrofluorometry under UV excitation. Evaluation of reactive oxygen species (ROS) release was performed using the 2′,7′-dichlorodihydrofluorescein diacetate (H2-DCFDA) staining and fluorescence emission measurement. VD3 (1, 10, 100 nM) or EB1089 (0.1, 1 and 10 nM) induces a decrease in autofluorescence emission intensity that can be attributed to the oxidation of the coenzyme nicotinamide adenine dinucleotide (phosphate) NAD(P)H into NAD(P)+. A dose-dependent increase (p
ISSN:1533-0346
1533-0338
DOI:10.1177/153303460400300409