Identification of Listeria monocytogenes in cattle meat using biochemical methods and amplification of the hemolysin gene

In Brazil and in other countries of the world, studies have been conducted to identify Listeria monocytogenes in cattle meat that is preferably consumed undercooked and, when marketed without meeting strict phytosanitary requirements, may cause outbreaks of listeriosis. In the such, foodborne outbre...

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Published in:Anais da Academia Brasileira de Ciências 2020, Vol.92 Suppl 1 (suppl 1), p.e20180557-e20180557
Main Authors: Silva, Alessandra S, Duarte, Elizabeth A A, Oliveira, Thiago A S DE, Evangelista-Barreto, Norma S
Format: Article
Language:English
Subjects:
Animals
Brazil
Cattle
DNA, Bacterial - analysis
Food Microbiology
Foodborne diseases
Hemolysin Proteins - analysis
Hemolysin Proteins - genetics
hly A gene
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
Listeriosis
meat products
Meat Products - microbiology
MULTIDISCIPLINARY SCIENCES
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Summary:In Brazil and in other countries of the world, studies have been conducted to identify Listeria monocytogenes in cattle meat that is preferably consumed undercooked and, when marketed without meeting strict phytosanitary requirements, may cause outbreaks of listeriosis. In the such, foodborne outbreaks, the methods used for the detection of the pathogen and the efficiency associated with them are crucial for the proper assessment. In this study, we used the techniques biochemical and molecular for identification of the L. monocytogenes isolated from 30 samples of the fresh beef, marketed in ten butchers' shop of the free-fair from a municipality from the Bahia, Brazil. The results obtained from biochemical tests (catalase, motility, β-hemolysis and carbohydrate fermentation), as well as PCR analysis for the hly gene (hemolysin production is an important factor in the pathogenesis of listeriosis) revealed that 50% of butchers shops presented bovine meat contaminated with bacteria of the Listeria sp. and confirmed that 54.16% of the analyzed meat samples were positive for L. monocytogenes. This study highlights the importance of microbiological surveillance in free-fair to minimize the exposure of consumers to this foodborne pathogen.
ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202020180557