Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers

This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses. Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic...

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Published in:Arquivos brasileiros de endocrinologia e metabologia 2014-04, Vol.58 (3), p.251-259
Main Authors: Abreu, Isabel Cristina Mallosto Emerich de, Guerra, Joyce Ferreira da Costa, Pereira, Renata Rebeca, Silva, Maísa, Lima, Wanderson Geraldo de, Silva, Marcelo Eustáquio, Pedrosa, Maria Lúcia
Format: Article
Language:English
Subjects:
Alanine Transaminase - blood
Animals
Antioxidants - analysis
Aspartate Aminotransferases - blood
Body Weight
Catalase - metabolism
Cholesterol - blood
Cholesterol, Dietary - adverse effects
Diet, High-Fat - adverse effects
ENDOCRINOLOGY & METABOLISM
Fatty Liver - etiology
Female
Glutathione - analysis
Hypercholesterolemia - etiology
Lipids - blood
Liver - enzymology
NADPH Oxidases - genetics
NADPH Oxidases - metabolism
Oxidative Stress
Rats, Inbred F344
Real-Time Polymerase Chain Reaction
RNA, Messenger - metabolism
Superoxide Dismutase - metabolism
Triglycerides - blood
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Summary:This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses. Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic diet (H). Those in group C were fed a standard diet (AIN-93M), and those of the group H were fed a hypercholesterolemic diet (25% soybean oil and 1% cholesterol). The hypercholesterolemic diet did not affect body weight, but resulted in the accumulation of lipids in the liver, increased serum activities of aminotransferases and cholesterol levels. Biomarker of lipid peroxidation (TBARS) and mRNA expression of NADPH oxidase subunits p22(phox) and p47(phox) were increased in the liver of animals in group H. Besides, the activity and expression of antioxidant enzymes were altered. The results show increased mRNA expression of NADPH oxidase subunits and changes in antioxidant enzyme activities in diet-induced hepatic steatosis.
ISSN:0004-2730
1677-9487
1677-9487
0004-2730
DOI:10.1590/0004-2730000002831