Investigating the effect of intracameral cefuroxime on oxidative stress and apoptosis in the rat cornea

• Published in: Arquivos brasileiros de oftalmologia 2019-07, Vol.82 (4), p.322-328
• Main Authors: Ozkan, Ufuk, Akal, Ali, Ozkan, Kudret, Yilmaz, Omer Faruk, Adıbelli, Fatih Mehmet
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Apoptosis - drug effects; Aryldialkylphosphatase - analysis; Carboxylic Ester Hydrolases - analysis; Caspase 3 - analysis; Caspase 8 - analysis; Cefuroxime - pharmacology; Cornea - drug effects; Cornea - metabolism; Cornea - pathology; Endothelium, Corneal - drug effects; Endothelium, Corneal - metabolism; Endothelium, Corneal - pathology; Immunohistochemistry; Injections, Intraocular; Male; OPHTHALMOLOGY; Oxidants - blood; Oxidative Stress - drug effects; Rats, Wistar; Reproducibility of Results
• ISSN: 0004-2749; 1678-2925; 1678-2925
• DOI: 10.5935/0004-2749.20190067

We examined the effect of intracameral administration of cefuroxime on oxidative stress and endothelial apoptosis in rat corneal tissue. In total, 30 rats were divided into 3 groups of 10 rats each (intracameral administration of cefuroxime 0.1 mg/0.01 mL (cefuroxime group); intracameral administration of balanced salt solution 0.01 mL (control group); or absence of intracameral injection (sham group). Corneal endothelial apoptosis was assessed by immunohistochemical analysis using caspase-3 and caspase-8. Total oxidant status, total antioxidant status, oxidative stress index, and paraoxonase and arylesterase levels were examined in corneal endothelial tissue and serum. Paraoxonase levels in the serum were significantly different between the sham and cefuroxime groups (p=0.027). A significant difference was also observed in total oxidant status levels between the cefuroxime and balanced salt solution groups (p=0.023). In addition, there were significant differences in total antioxidant status levels in corneal tissue between the cefuroxime and sham groups (p