Comparative analysis of biofilm formation by Candida albicans and Candida krusei in different types of contact lenses

• Published in: Arquivos brasileiros de oftalmologia 2022-01, Vol.85 (3), p.235-239
• Main Authors: Fritsch, Laura Nagy, Dias, Amanda Latercia Tranches, Silva, Naiara Chaves, Fernandes, Geraldo José Medeiros, Ribeiro, Flávia Beatriz de Andrade Oliveira
• Format: Article
• Language: English
• Subjects: OPHTHALMOLOGY
• ISSN: 0004-2749; 1678-2925; 1678-2925
• DOI: 10.5935/0004-2749.20220033

OBJECTIVETo evaluate the Candida krusei and Candida albicans biofilm formation abilities on 5 different types of contact lenses and compare their metabolic activities and biomass. METHODSAfter biofilm formation by both the test species, their metabolic activity was assessed by the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay with menadione, while the biomass was determined by staining with 0.4% crystal violet dye for further statistical analysis. RESULTSBoth the Candida species could form biofilms on different types of contact lenses, with greater metabolic activities and lower biomass formation in rigid gas permeable lenses. CONCLUSIONBiofilm formation with greater metabolic activity and greater biomass were expected on soft contact lenses considering their surface hydrophobicity. However, the results demonstrated a greater metabolic activity on rigid contact lenses. This result has a great significance with regards to the increasing risk of microbial keratitis, although further studies are warranted to better elucidate the formation of biofilms on different types of contact lens materials in the future.