Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population

In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 1989-10, Vol.31 (5), p.336-340
Main Authors: WEINBERG, A, FINK, M. C. D. S, TAKIMOTO, S, ISHIDA, M. A, SOUZA, M. C. O
Format: Article
Language:English
Subjects:
Adenoviridae - immunology
Adenovirus Infections, Human - diagnosis
Antibodies, Anti-Idiotypic - analysis
Antibodies, Viral - analysis
Biological and medical sciences
Complement Fixation Tests
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Human viral diseases
Humans
Immunity, Maternally-Acquired
Immunoglobulin M - analysis
Infant
Infectious diseases
Medical sciences
Miscellaneous
TROPICAL MEDICINE
Viral diseases
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Summary:In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/s0036-46651989000500007