DOT-ELISA for detection of anti-Cysticercus cellulosae antibodies in human cerebrospinal fluid using a new solid-phase (resin-treated polyester fabrics). Preliminary report

• Published in: Revista do Instituto de Medicina Tropical de São Paulo 1990-09, Vol.32 (5), p.355-359
• Main Authors: VAZ, A. J, FERREIRA, A. W, CAMARGO, M. E, NAKAMURA, P. M, CAMARGO, E. D
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - cerebrospinal fluid; Biological and medical sciences; Central Nervous System Diseases - cerebrospinal fluid; Cysticercosis; Cysticercosis - cerebrospinal fluid; Cysticercus - immunology; Diseases caused by cestodes; Helminthic diseases; Humans; Immunoblotting; Infectious diseases; Medical sciences; Parasitic diseases; Resins, Synthetic; Sensitivity and Specificity; TROPICAL MEDICINE
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46651990000500008

A dot enzyme-linked immunosorbent assay (DOT-ELISA) was developed to detect specific antibodies in cerebrospinal fluid (CSF) for human neurocysticercosis immunodiagnosis, with Cysticercus cellulosae antigen dotted on a new solid-phase. This was represented by sheets of a synthetic polyester fabric impregnated with a polymerized resin (N-methylol-acrylamide). A very stable preparation was thus obtained, the antigen being covalently bound by cross-linking with free N-methylol groups on the resin. Since robust, no special care was necessary for handling the solid-phase. The test could be performed at room-temperature. From 30 CSF samples assayed, 14 were positive, from a group of 15 cases of neurocysticercosis, with titers from 1 to 128; 15 other samples, from normals or other neurological diseases, were all negative. Test characteristics seem to indicate it as adequate for epidemiological surveys. A more detailed study on sensitivity, specificity, reproducibility and the use in serum samples is being conducted.