Epidemiological, survey of Trypanosoma cruzi infection in north-eastern Brazil using different diagnostic methods

A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELI...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 1991-06, Vol.33 (3), p.193-198
Main Authors: FIGUEREDO-SILVA, J, KANEDA, Y, TACHIBANA, H, FURUSHIMA, R, TATENO, S, CORREIA-LIMA, F. G, BENTO, D. N. C
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Animals
Biological and medical sciences
Brazil - epidemiology
Chagas Disease - diagnosis
Chagas Disease - epidemiology
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique
Human protozoal diseases
Humans
Infant
Infant, Newborn
Infectious diseases
Male
Medical sciences
Middle Aged
Parasitic diseases
Prevalence
Protozoal diseases
TROPICAL MEDICINE
Trypanosomiasis
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Summary:A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/S0036-46651991000300005