Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226)...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 1995-08, Vol.37 (4), p.357-359
Main Authors: de Souza, V A, Sumita, L M, Otsubo, M E, Takei, K, Pannuti, C S
Format: Article
Language:English
Subjects:
Antibodies, Viral - blood
Enzyme-Linked Immunosorbent Assay - methods
Humans
Immunoglobulin G - blood
Rubella - diagnosis
Rubella - immunology
Rubella virus - immunology
TROPICAL MEDICINE
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Summary:A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.
ISSN:0036-4665
1678-9946
0036-4665
DOI:10.1590/s0036-46651995000400013