Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens

• Published in: Revista do Instituto de Medicina Tropical de São Paulo 1996-12, Vol.38 (6), p.391-396
• Main Authors: Vaz, A J, Nakamura, P M, Camargo, M E, Camargo, E D, Ferreira, A W
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - cerebrospinal fluid; Antigens, Helminth; Cysticercosis - diagnosis; Cysticercus - immunology; Enzyme-Linked Immunosorbent Assay - methods; Female; Mice; Mice, Inbred BALB C; Resins, Synthetic; Swine; TROPICAL MEDICINE
• ISSN: 0036-4665; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46651996000600001

A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis.