Comparison among tomato juice agar with other three media for differentiation of Candida dubliniensis from Candida albicans

The purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between C. dubliniensis and C. albicans. After 48 hours of incubation at 30 degre...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 2006-05, Vol.48 (3), p.119-121
Main Authors: Alves, Sydney Hartz, de Loreto, Erico Silva, Linares, Carlos Eduardo, Silveira, Carolina P, Scheid, Liliane A, Pereira, Daniela I Brayer, Santuario, Janio Morais
Format: Article
Language:English
Subjects:
Agar - chemistry
Candida - classification
Candida - growth & development
Candida - isolation & purification
Candida albicans - classification
Candida albicans - growth & development
Culture Media - chemistry
Lycopersicon esculentum
Mycological Typing Techniques - methods
Spores, Fungal - growth & development
TROPICAL MEDICINE
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Summary:The purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between C. dubliniensis and C. albicans. After 48 hours of incubation at 30 degrees C all 26 (100%) C. dubliniensis isolates tested produced chlamydospores on tomato juice agar as well as in the other three media evaluated. However, when we inoculated all media with C. albicans, the absence of chlamydospores became resulting in the following percents: tomato juice agar (92.47%), niger seed agar (96.7%), casein agar (91.39%), and sunflower seed agar (96.7%). These results indicate that tomato juice agar is another medium which can also be used in the first phenotypic differentiation between C. dubliniensis and C. albicans.
ISSN:0036-4665
1678-9946
0036-4665
1678-9946
DOI:10.1590/S0036-46652006000300001