Natural infection of phlebotomines (Diptera: Psychodidae) in a visceral-leishmaniasis focus in Mato Grosso do Sul, Brazil

The main purpose of this study was to investigate natural infection by Leishmania in phlebotomine females in a visceral-leishmaniasis focus in Antonio João county in Mato Grosso do Sul State, Brazil. Between June and October 2003, the digestive tracts of 81 females captured in Aldeia Campestre, Alde...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2007-03, Vol.49 (2), p.119-122
Main Authors: do Nascimento, João Cezar, de Paiva, Byanca Regina, dos Santos Malafronte, Rosely, Fernandes, Wedson Desidério, Galati, Eunice Aparecida Bianchi
Format: Article
Language:English
Subjects:
Animals
Brazil
DNA, Ribosomal - analysis
Female
Humans
Insect Vectors - classification
Insect Vectors - parasitology
Leishmania - classification
Leishmania - genetics
Leishmania - isolation & purification
Leishmaniasis, Visceral - transmission
Polymerase Chain Reaction
Psychodidae - classification
Psychodidae - parasitology
TROPICAL MEDICINE
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Summary:The main purpose of this study was to investigate natural infection by Leishmania in phlebotomine females in a visceral-leishmaniasis focus in Antonio João county in Mato Grosso do Sul State, Brazil. Between June and October 2003, the digestive tracts of 81 females captured in Aldeia Campestre, Aldeia Marangatu and Povoado Campestre were dissected. The females were separated by species, location, area and date of capture into 13 groups and kept in ethanol 70%. To identify the Leishmania species using the PCR technique, amplifications of the ribosomal-DNA (rDNA) and mini-exon genes were analyzed. Of the 81 specimens, 77 (95%) were Lutzomyia longipalpis, making this the most common species; only one specimen of each of the species Brumptomyia avellari, Evandromyia cortelezzii, Evandromyia lenti and Nyssomyia whitmani was found. Trypanosomatids were identified in eight of the nine groups of Lutzomyia longipalpis (10.39%) one group from Aldeia Campestre, one from Aldeia Marangatu and six from Povoado Campestre; of the eight groups, one from Aldeia Marangatu and another, with promastigotes forms also confirmed by dissection (1.23%) from Povoado Campestre, were identified by PCR as Leishmania chagasi (2.6%). The other groups gave negative results. These findings indicate that there is a high risk of leishmaniasis transmission in this area.
ISSN:0036-4665
1678-9946
0036-4665
1678-9946
DOI:10.1590/s0036-46652007000200011