Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

• Published in: Revista da Sociedade Brasileira de Medicina Tropical 2007-05, Vol.40 (3), p.264-267
• Main Authors: da Silva, Heloísa Helena Garcia, Geris, Regina, Rodrigues Filho, Edson, Rocha, Cleonice, da Silva, Ionizete Garcia
• Format: Article
• Language: English
• Subjects: Aedes; Animals; Aquatic insects; Balsams - chemistry; Bioassays; Brazil; Chromatography, Thin Layer; Insecticides; Larva - drug effects; Larvae; Lethal Dose 50; Magnetic Resonance Spectroscopy; Mass Spectrometry; Medicinal plants; Mosquitoes; NMR; Nuclear magnetic resonance; Relative humidity; Thin layer chromatography; TROPICAL MEDICINE
• ISSN: 0037-8682; 1678-9849; 0037-8682; 1678-9849
• DOI: 10.1590/s0037-86822007000300002

Oil-resin fractions from Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) were evaluated for larvicidal activity on third larval instars of Aedes aegypti, in searching for alternative control methods for this mosquito. The bioactive fractions were chemically monitored by thin-layer chromatography, (1)H and 13C nuclear magnetic resonance and mass spectrometry. Bioassays were performed using five repetitions, at a temperature of 28+/-1 degrees C, relative humidity of 80+/- 5% and light and dark cycles of 12h. Mortality was indicated by darkening of the cephalic capsule after 24h of exposure of the larvae to the solutions. The most active fractions were CRM1-4 (sesquiterpenes) and CRM5-7 (labdane diterpenes), which showed LC50 values of 0.2 and 0.8 ppm, respectively.