Ex vivo T-lymphocyte chemokine receptor phenotypes in patients with chronic Chagas disease

• Published in: Revista da Sociedade Brasileira de Medicina Tropical 2017-09, Vol.50 (5), p.689-692
• Main Authors: Miranda, Matheus Barbosa de, Melo, Adriene Siqueira de, Almeida, Mariana Silva, Marinho, Silvia Martins, Oliveira, Junior, Wilson, Gomes, Yara de Miranda
• Format: Article
• Language: English
• Subjects: Aged; Cardiomyopathy; CC chemokine receptors; CCR1 protein; CCR5 protein; CD4 antigen; CD4-Positive T-Lymphocytes - chemistry; CD8 antigen; CD8-Positive T-Lymphocytes - chemistry; Chagas Cardiomyopathy - blood; Chagas Cardiomyopathy - pathology; Chagas disease; Chemokine receptors; Chemokines; Chronic Chagas disease; CXCR2 protein; CXCR3 protein; CXCR4 protein; CXCR5 protein; Cytometry; Female; Flow Cytometry; Gene Expression; Heart diseases; Humans; Identification methods; Inflammation; Lymphocytes; Lymphocytes T; Male; Middle Aged; Molecular chains; Pathogenesis; Patients; Phenotype; Receptors; Receptors, CCR - blood; Reference Values; Severity of Illness Index; Statistics, Nonparametric; Target recognition; TROPICAL MEDICINE; Vector-borne diseases
• ISSN: 0037-8682; 1678-9849; 1678-9849
• DOI: 10.1590/0037-8682-0025-2017

Elucidating the molecules involved in the inflammatory process of chronic Chagas disease may allow identification of treatment targets. The ex vivo phenotypic expression of chemokine receptors CCR1, CCR3, CCR4, CCR5, CXCR2, CXCR3, CXCR4, and CXCR5 on the CD4+ and CD8+ T-cells of patients with chronic Chagas cardiomyopathy of varying severity was evaluated using flow cytometry. Differential expression of CD4+CCR3+ and CD8+CCR4+ T-cells was observed in patients with mild cardiac involvement compared, respectively, with patients with severe cardiac and asymptomatic forms of Chagas disease. These receptors are possibly involved in the pathogenesis of chronic Chagas cardiomyopathy.