First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil

Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify...

Full description

Saved in:
Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2017-01, Vol.112 (1), p.75-78
Main Authors: Araujo-Pereira, Thais de, Pita-Pereira, Daniela de, Boité, Mariana Côrtes, Melo, Myllena, Costa-Rego, Taiana Amancio da, Fuzari, Andressa Alencastre, Brazil, Reginaldo Peçanha, Britto, Constança
Format: Article
Language:English
Subjects:
Acre
Animals
Brazil
DNA, Protozoan - genetics
Insect Vectors - classification
Insect Vectors - parasitology
Leishmania - classification
Leishmania - isolation & purification
Leishmania Viannia
Leishmaniasis, Cutaneous - transmission
molecular diagnosis
Multiplex Polymerase Chain Reaction
PARASITOLOGY
Psychodidae - classification
Psychodidae - parasitology
sandfly
Short Communication
TROPICAL MEDICINE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12 out of 173 sandflies captured in the municipality of Rio Branco, Acre state, revealing a positivity of 6.94%. By sequencing the Leishmania 234 bp-hsp70 amplified products from positive samples, infection by L. (V.) braziliensis was confirmed in five sandflies: one Evandromyia saulensis, three Trichophoromyia auraensis and one Pressatia sp. The finding of L. (Viannia) DNA in two Ev. saulensis corresponds to the first record of possible infection associated with this sandfly. Moreover, our study reveals for the first time in Brazil, Th. auraensis and Pressatia sp. infected by L. (Viannia) parasites.
ISSN:0074-0276
1678-8060
1678-8060
0074-0276
DOI:10.1590/0074-02760160283