Morphologic study of the effect of iron on pseudocyst formation in Trichomonas vaginalis and its interaction with human epithelial cells

• Published in: Memórias do Instituto Oswaldo Cruz 2017-10, Vol.112 (10), p.664-673
• Main Authors: Dias-Lopes, Geovane, Saboia-Vahia, Leonardo, Margotti, Eliane Trindade, Fernandes, Nilma de Souza, Castro, Cássia Luana de Faria, Oliveira, Junior, Francisco Odencio, Peixoto, Juliana Figueiredo, Britto, Constança, Silva, Filho, Fernando Costa E, Cuervo, Patricia, Jesus, José Batista de
• Format: Article
• Language: English
• Subjects: Epithelial Cells - microbiology; HeLa Cells; human epithelial cells; Humans; iron; Iron Chelating Agents - pharmacology; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; morphological transformation; PARASITOLOGY; pseudocyst; Time Factors; Trichomonas vaginalis; Trichomonas vaginalis - drug effects; Trichomonas vaginalis - ultrastructure; trophozoites; TROPICAL MEDICINE
• ISSN: 0074-0276; 1678-8060; 1678-8060; 0074-0276
• DOI: 10.1590/0074-02760170032

Trichomonas vaginalis is the aetiological agent of human trichomoniasis, which is one of the most prevalent sexually transmitted diseases in humans. Iron is an important element for the survival of this parasite and the colonisation of the host urogenital tract. In this study, we investigated the effects of iron on parasite proliferation in the dynamics of pseudocyst formation and morphologically characterised iron depletion-induced pseudocysts. We performed structural and ultrastructural analyses using light microscopy, scanning electron microscopy and transmission electron microscopy. It was observed that iron depletion (i) interrupts the proliferation of T. vaginalis, (ii) induces morphological changes in typical multiplicative trophozoites to spherical non-proliferative, non-motile pseudocysts, and (iii) induces the arrest of cell division at different stages of the cell cycle; (iv) iron is the fundamental element for the maintenance of typical trophozoite morphology; (v) pseudocysts induced by iron depletion are viable and reversible forms; and, finally, (vi) we demonstrated that pseudocysts induced by iron depletion are able to interact with human epithelial cells maintaining their spherical forms. Together, these data suggest that pseudocysts could be induced as a response to iron nutritional stress and could have a potential role in the transmission and infection of T. vaginalis.